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STUDY ON COMPARISON OF ANTIMICROBIAL ACTIVITY BETWEEN HORSERADISH(ARMORACIA RUSTICANA) ROOT EXTRACTS AND SYNTHETIC ALLYL ISOTHIOCYANATE AGAINST ORAL PATHOGENIC MICROORGANISMS

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ÀÌÁÖÇö ( Lee Ju-Hyun ) - °­¸ª´ëÇб³ Ä¡°ú´ëÇÐ ¼Ò¾ÆÄ¡°úÇб³½Ç ¹× ±¸°­°úÇבּ¸¼Ò
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Abstract

¼­¾ç»ê °íÃß³ÃÀÌ (Horseradish, Armoracia rusticana)ÀÇ Ç×±Õ ¼ººÐ Áß ÁÖ ¼ººÐÀÎ Allyl isothiocyanate(AIT)´Â õ¿¬¹°À» ÀÌ¿ëÇÑ ÃßÃâÀ̳ª À¯±âÇÕ¼ºÀ» ÅëÇؼ­ ¾òÀ» ¼ö ÀÖÀ¸¸ç, ÀÌÁ¦±îÁö »ç¿ëµÇ°í ÀÖ´Â Ç×±ÕÁ¦Ç° ¹× ½Äǰ÷°¡¹°µéÀº ÁÖ·Î À¯±âÇÕ¼º¹ý¿¡ ÀÇÇؼ­ ¾ò¾îÁ³´Ù. ÀÌÀü ¿¬±¸¿¡¼­ ¼­¾ç»ê °íÃß³ÃÀÌ Ãµ¿¬ÃßÃâ¹°ÀÇ ´Ù¾çÇÑ ±¸°­ ³» ¹Ì»ý¹°¿¡ ´ëÇÑ Ç×±ÕÈ°¼ºÀº º¸°íµÈ ¹Ù ÀÖÀ¸³ª À¯±âÇÕ¼ºÇÑ AIT¿ë¾×ÀÇ ±¸°­ ³» ¹Ì»ý¹°¿¡ ´ëÇÑ Ç×±ÕÈ°¼ºÀº ¾ÆÁ÷±îÁö ¿¬±¸µÈ ¹Ù°¡ ¾ø´Ù. ÀÌ¿¡ º» ¿¬±¸¿¡¼­´Â À¯±âÇÕ¼ºÀ» ÅëÇØ ¾òÀº AIT ¿ë¾×°ú ¼­¾ç»ê °íÃß³ÃÀÌ »Ñ¸® õ¿¬ÃßÃâ¹°ÀÇ ±¸°­ ³» ¹Ì»ý¹°¿¡ ´ëÇÑ Ç×±ÕÈ°¼ºÀ» Æò°¡ÇÏ¿´´Ù. Ç×±ÕÈ°¼ºÀ» Æò°¡Çϱâ À§ÇØ ÃÖ¼Ò¾ïÁ¦³óµµ¿Í ÃÖ¼Ò »ì±Õ³óµµ¸¦ ÃøÁ¤ÇÏ¿´À¸¸ç, ±× °á°ú´Â ´ÙÀ½°ú °°´Ù. 1. º» ¿¬±¸¿¡ »ç¿ëµÈ 7Á¾ÀÇ ±¸°­ ³» ¹Ì»ý¹°¿¡ ´ëÇÑ ¼­¾ç»ê °íÃß³ÃÀÌ »Ñ¸® õ¿¬ÃßÃâ¹°ÀÇ ÃÖ¼Ò¾ïÁ¦³óµµ´Â ¾à 1171,750 ppm(0.01170.175%)À̾úÀ¸¸ç, À¯±âÇÕ¼ºÇÑ AIT ¿ë¾×ÀÇ ÃÖ¼Ò¾ïÁ¦³óµµ´Â ¾à 3443,000 ppm(0.03440.3%)À¸·Î ¸ðµç ±ÕÁÖ¿¡ ´ëÇØ Ç×±ÕÈ°¼ºÀ» ³ªÅ¸³»¾ú´Ù. 2. º» ¿¬±¸¿¡ »ç¿ëµÈ 7Á¾ÀÇ ±¸°­ ³» ¹Ì»ý¹°¿¡ ´ëÇÑ ¼­¾ç»ê °íÃß³ÃÀÌ »Ñ¸® õ¿¬ÃßÃâ¹°ÀÇ ÃÖ¼Ò»ì±Õ³óµµ´Â ¾à 625.26,000 ppm(0.062520.6%)À̾úÀ¸¸ç, À¯±âÇÕ¼ºÇÑ AIT¿ë¾×ÀÇ ÃÖ¼Ò»ì±Õ³óµµ´Â ¾à 1,7507,000 ppm(0.1750.7%)À̾ú´Ù.

Allyl isothiocyanate (AIT), the principle ingredient of antimicrobial ingredients from horseradish root, can be prepared from extracts of horseradish root or synthetic method. It is reported that the horseradish root extract has the antimicrobial effect against various oral microorganisms, while there is no further study about the antimicrobial effect against the oral microorganisms of synthetic AIT derived from synthetic method. The aim of the study is to compare the difference of the antimicrobial effect between horseradish root extracts and synthetic AIT. To evaluate the antimicrobial effect, we measured the minimum inhibitory concentration(MIC) and minimum bactericidal concentration (MBC), and the results are like following. 1. The MIC of horseradish root extract against 7 kinds of oral pathogenic microorganisms is about 1171,750 ppm(0.01170.175%), and the MIC of the synthetic AIT is about 3443,000 ppm(0.03440.3%), which have the antimicrobial effects against all kinds of microorganisms. 2. The MBC of the horseradish root extracts against the 7 kinds of oral microorganisms is about 625.26,000 ppm(0.062520.6%), and the MBC of the synthetic AIT is about 1,7507,000 ppm(0.1750.7%), which have the antimicrobial effects against all kinds of microorganisms.

Å°¿öµå

°íÃß³ÃÀÌ;õ¿¬ÃßÃâ¹°;¿Í»çºñ;Ç×±ÕÈ°¼º
Horseradish(Armoracia rusticana);Natural extract;Allyl isothiocyanate(AIT);Wasabi;Antimicrobial activity

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